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BioMed Research International
Volume 2014, Article ID 104230, 9 pages
Research Article

Effect of Low-Level Laser on Bone Defects Treated with Bovine or Autogenous Bone Grafts: In Vivo Study in Rat Calvaria

1Hospital for Rehabilitation of Craniofacial Anomalies, University of São Paulo, Brazil
2Bauru School of Dentistry, USP, Brazil
3Faculdade de Odontologia de Bauru (FOB), Universidade de São Paulo (USP), Alameda Dr. Octávio Pinheiro Brisolla 9-75, Vila Universitária, 17012 901 Bauru, SP, Brazil
4Department of Prosthodontics, Bauru School of Dentistry, USP, Brazil

Received 28 February 2014; Revised 17 April 2014; Accepted 12 May 2014; Published 28 May 2014

Academic Editor: David M. Dohan Ehrenfest

Copyright © 2014 Mércia J. S. Cunha et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Objective. The purpose of this study was to histologically evaluate the effect of low-level laser (LLL) on the healing of critical size defects (CSD) in rat calvaria, filled with autogenous or inorganic bovine bone grafts. Methods. Sixty rats were divided into 6 groups : C (control—filled with blood clot), LLL (low-level laser—GaAlAs, 780 nm, 100 mW, 210 J/cm2, 0.05 cm2; 6 J/point), AB (autogenous bone), ABL (autogenous bone + low-level laser), OB (inorganic bovine bone), and OBL (inorganic bovine bone + LLL). Material and Methods. The animals were killed after 30 days. Histological and histometric analyses were performed by light microscopy. Results. The groups irradiated with laser, LLL (47.67% ± 8.66%), ABL (39.15% ± 16.72%), and OBL (48.57% ± 28.22%), presented greater area of new bone formation than groups C (9.96% ± 4.50%), AB (30.98% ± 16.59%), and OB (11.36% ± 7.89%), which were not irradiated. Moreover, they were significantly better than group C (Kruskal-Wallis test followed by Dunn test, . Conclusion. The laser accelerated the healing of bone defects and the resorption of particles of the graft material.