Emodin Augments Cisplatin Cytotoxicity in Platinum-Resistant Ovarian Cancer Cells via ROS-Dependent MRP1 Downregulation

Figure 3

Production of ROS in response to cDDP and emodin. COC1/DDP cells were treated with 50 μM emodin, 33 μM cDDP, 10 mM NAC, 12.5, 25, or 50 μM emodin + 33 μM cDDP, or 50 μM emodin + 33 μM cDDP + 10 mM NAC for 24 h and then incubated with DCFDA for 15 min. Cellular ROS level was reflected by DCF intensity and expressed as a fold change of the DCF intensity relative to that in the untreated control cells. Values represent mean ± S.D. of three separate experiments performed in duplicate. versus cDDP-only treated cells; versus the 30 μM DDP + 50 μM emodin cotreated cells.