Lipolytic Potential of Aspergillus japonicus LAB01: Production, Partial Purification, and Characterisation of an Extracellular Lipase

Table 5

Effect of organic solvents on the activity/stability of the lipase from A. japonicus LAB01.

Solvents	Residual activity (%)
Hexane	137.1 ± 4.71
Toluene	100.9 ± 4.70
Acetonitrile	0
Acetone	0
Methanol (25% v/v)	98.48 ± 0.97
Methanol (50% v/v)	108.09 ± 0.34
Methanol (75% v/v)	13.10 ± 0.87
Ethanol (25% v/v)	90.71 ± 3.89
Ethanol (50% v/v)	12.65 ± 1.65
Ethanol (75% v/v)	4.03 ± 0.03
Isopropanol (25% v/v)	70.65 ± 0.25
Isopropanol (50% v/v)	2.05 ± 0.19
Isopropanol (75% v/v)	1.64 ± 0.06

Lipase samples were incubated with organic solvents at 30°C for 90 min. The remaining lipase activity relative to the non-solvent containing control was determined on pNPP substrate at pH 8.0 and 37°C. Values are the average of assays carried out in triplicate.