Recombinant nAG (a Salamander-Derived Protein) Decreases the Formation of Hypertrophic Scarring in the Rabbit Ear Model

Table 1

Primers and probes used for real-time PCR.

Gene	Gene accession number	Primer and probe sequence	Amplicon size (bp)
Rab. COL1 F.P.	NM_001195668 XM_002713800	CCCAACCAAGGATGCACTAT	126
Rab. COL1 Probe		5′-FAM, TCTCTACTGGCGAAACCTGCATCC, BHQ1-3′
Rab. COL1 R.P.		CTTGGCCTTGGAGCTCTTATAC
Rab. COL3 F.P.	S83371.1	CATTGGCCCTGTTTGCTTT	110
Rab. COL3 Probe		5′-FAM, AAACCAACCTCTTCCTGAAGCCC, BHQ1-3′
Rab. COL3 R.P.		CACTTGTACTGGTTGACAAGATTAG
Rab. MMP1 F.P.	NM_001171139	GCAGAATGAGCTACCAGGATAC	89
Rab. MMP1 Probe		5′-FAM, AGGACATTCACAGCTCCTTTGGCT, BHQ1-3′
Rab. MMP1 R.P.		CAGAAACAGCAGCGTCAATATG
Rab. GAPDH F.P.	NM_001082253.1	ATGGCCTCCAAGGAGTAAGA	149
Rab. GAPDH Probe		5′-HEX, CAGTCCCACCACGCTGAGAATCTG, BHQ1-3′
Rab. GAPDH R.P.		CTGGGATGGAAACTGTGAAGAG