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BioMed Research International
Volume 2014 (2014), Article ID 123079, 5 pages
http://dx.doi.org/10.1155/2014/123079
Research Article

Peripheral Blood WT1 Expression Predicts Relapse in AML Patients Undergoing Allogeneic Stem Cell Transplantation

1Unit of Blood Disease and Stem Cell Transplantation, Department of Clinical and Experimental Sciences, University of Brescia, AO Spedali Civili di Brescia, P.le Ospedali Civili 1, 25123 Brescia, Italy
2Chair of Biochemistry, Department of Molecular and Translational Medicine, Univeristy of Brescia, Laboratorio Analisi, AO Spedali Civili di Brescia, P.le Ospedali Civili 1, 25123 Brescia, Italy
3Division of Hematology, AO Spedali Civili di Brescia, P.le Ospedali Civili 1, 25123 Brescia, Italy

Received 17 June 2014; Accepted 23 July 2014; Published 17 August 2014

Academic Editor: Alessandro Isidori

Copyright © 2014 Michele Malagola et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. V. Válková, J. Polák, M. Marková et al., “Minimal residual disease detectable by quantitative assessment of WT1 gene before allogeneic stem cell transplantation in patients in first remission of acute myeloid leukemia has an impact on their future prognosis,” Clinical Transplantation, vol. 27, pp. E21–E29, 2013. View at Google Scholar
  2. J. Polák, H. Hájková, J. Maalaufová-Soukupová et al., “Estimation of molecular upper remission limit for monitoring minimal residual disease in peripheral blood of acute myeloid leukemia patients by WT1 expression,” Experimental and Therapeutic Medicine, vol. 3, pp. 129–133, 2012. View at Google Scholar
  3. H. Miwa, M. Beran, and G. F. Saunders, “Expression of the Wilms' tumor gene (WT1) in human leukemias,” Leukemia, vol. 6, no. 5, pp. 405–409, 1992. View at Google Scholar · View at Scopus
  4. K. Inoue, H. Ogawa, Y. Sonoda et al., “Aberrant overexpression of the Wilms tumor gene (WT1) in human leukemia,” Blood, vol. 89, no. 4, pp. 1405–1412, 1997. View at Google Scholar · View at Scopus
  5. M. Weisser, W. Kern, S. Rauhut et al., “Prognostic impact of RT-PCR-based quantification WT1 gene expression during MRD monitoring of acute myeloid leukemia,” Leukemia, vol. 19, no. 8, pp. 1416–1423, 2005. View at Publisher · View at Google Scholar · View at Scopus
  6. D. Cilloni, A. Renneville, F. Hermitte et al., “Real-time quantitative polymerase chain reaction detection of minimal residual disease by standardized WT1 assay to enhance risk stratification in acute myeloid leukemia: a European LeukemiaNet Study,” Journal of Clinical Oncology, vol. 27, no. 31, pp. 5195–5201, 2009. View at Publisher · View at Google Scholar · View at Scopus
  7. J. F. Nomdedéu, M. Hoyos, M. Carricondo et al., “Bone marrow WT1 levels at diagnosis, post-induction and post-intensification in adult de novo AML,” Leukemia, vol. 27, pp. 2157–2164, 2013. View at Publisher · View at Google Scholar · View at Scopus
  8. G. Rossi, M. M. Minervini, L. Melillo et al., “Predictive role of minimal residual disease and log clearance in acute myeloid leukemia: a comparison between multiparameter flow cytometry and Wilm's tumor 1 levels,” Annals of Hematology, vol. 93, no. 7, pp. 1149–1157, 2014. View at Publisher · View at Google Scholar
  9. P. Paschka, G. Marcucci, A. S. Ruppert et al., “Wilms' tumor 1 gene mutations independently predict poor outcome in adults with cytogenetically normal acute myeloid leukemia: a cancer and leukemia group B study,” Journal of Clinical Oncology, vol. 26, no. 28, pp. 4595–4602, 2008. View at Publisher · View at Google Scholar · View at Scopus
  10. J. X. Gray, L. McMillen, P. Mollee et al., “WT1 expression as a marker of minimal residual disease predicts outcome in acute myeloid leukemia when measured post-consolidation,” Leukemia Research, vol. 36, no. 4, pp. 453–458, 2012. View at Publisher · View at Google Scholar · View at Scopus
  11. S. Pozzi, S. Geroldi, E. Tedone et al., “Leukaemia relapse after allogeneic transplants for acute myeloid leukaemia: predictive role of WT1 expression,” British Journal of Haematology, vol. 160, no. 4, pp. 503–509, 2013. View at Publisher · View at Google Scholar · View at Scopus
  12. M. Kwon, C. Martínez-Laperche, M. Infante et al., “Evaluation of minimal residual disease by real-time quantitative PCR of Wilms' tumor 1 expression in patients with acute myelogenous leukemia after allogeneic stem cell transplantation: correlation with flow cytometry and chimerism,” Biology of Blood and Marrow Transplantation, vol. 18, no. 8, pp. 1235–1242, 2012. View at Publisher · View at Google Scholar · View at Scopus
  13. E. Barragán, J. Cervera, P. Bolufer et al., “Prognostic implications of Wilms' tumor gene (WT1) expression in patients with de novo acute myeloid leukemia,” Haematologica, vol. 89, no. 8, pp. 926–933, 2004. View at Google Scholar · View at Scopus
  14. A. Candoni, M. Tiribelli, E. Toffoletti et al., “Quantitative assessment of WT1 gene expression after allogeneic stem cell transplantation is a useful tool for monitoring minimal residual disease in acute myeloid leukemia,” European Journal of Haematology, vol. 82, no. 1, pp. 61–68, 2009. View at Publisher · View at Google Scholar · View at Scopus
  15. A. Candoni, E. Toffoletti, R. Gallina et al., “Monitoring of minimal residual disease by quantitative WT1 gene expression following reduced intensity conditioning allogeneic stem cell transplantation in acute myeloid leukemia,” Clinical Transplantation, vol. 25, no. 2, pp. 308–316, 2011. View at Publisher · View at Google Scholar · View at Scopus
  16. H. Ogawa, H. Tamaki, K. Ikegame et al., “The usefulness of monitoring WT1 gene transcripts for the prediction and management of relapse following allogeneic stem cell transplantation in acute type leukemia,” Blood, vol. 101, no. 5, pp. 1698–1704, 2003. View at Publisher · View at Google Scholar · View at Scopus
  17. X. S. Zhao, C. H. Yan, D. H. Liu et al., “Combined use of WT1 and flow cytometry monitoring can promote sensitivity of predicting relapse after allogeneic HSCT without affecting specificity,” Annals of Hematology, vol. 92, no. 8, pp. 1111–1119, 2013. View at Publisher · View at Google Scholar · View at Scopus
  18. J. Yoon, H. Kim, S. Shin et al., “Serial measurement of WT1 expression and decrement ratio until hematopoietic cell transplantation as a marker of residual disease in patients with cytogenetically normal acute myelogenous leukemia,” Biology of Blood and Marrow Transplantation, vol. 19, no. 6, pp. 958–966, 2013. View at Publisher · View at Google Scholar · View at Scopus
  19. X.-S. Zhao, S. Jin, H.-H. Zhu et al., “Wilms tumor gene 1 expression: an independent acute leukemia prognostic indicator following allogeneic hematopoietic SCT,” Bone Marrow Transplantation, vol. 47, no. 4, pp. 499–507, 2012. View at Publisher · View at Google Scholar · View at Scopus
  20. H. Döhner, E. H. Estey, S. Amadori et al., “Diagnosis and management of acute myeloid leukemia in adults: recommendations from an international expert panel, on behalf of the European LeukemiaNet,” Blood, vol. 115, no. 3, pp. 453–474, 2010. View at Publisher · View at Google Scholar · View at Scopus
  21. E. R. Kaplan and P. Mejer, “Non parametric estimation from incomplete observation,” Journal of the American Statistical Association, vol. 53, pp. 457–481, 1958. View at Google Scholar