Research Article

Effect of Angiotensin II and Small GTPase Ras Signaling Pathway Inhibition on Early Renal Changes in a Murine Model of Obstructive Nephropathy

Figure 2

Effect of losartan administration on UUO-induced Ras pathway activation and fibrotic changes analyzed by Western blot. Protein expression of Ras (a), ERK1/2 (b), Akt ((c) and (d)), fibronectin (e), and α-SMA (f) detected by immunoblotting. Activation of Ras and ERK1/2 were measured as the ratio phosphorylated (p)/total proteins. Bars represent the mean ± SEM of the optical density measured in nonobstructed (NO) and obstructed (O) kidney samples of saline ( ) and losartan- (Los 40 mg/kg; ) treated animals. and versus NO vehicle-treated kidneys of UUO mice.
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