Research Article

Entamoeba histolytica and E. dispar Calreticulin: Inhibition of Classical Complement Pathway and Differences in the Level of Expression in Amoebic Liver Abscess

Figure 5

Confocal microscopy assay: colocation of EhCRT or EdCRT and human C1q. Panels (a) and (b) represent different patterns of immunodetection when using E. histolytica or E. dispar trophozoites, respectively; A: rabbit anti-EhCRT and Alexa Fluor 350-conjugated secondary antibody; B: trophozoites reacted with mouse anti-human C1q and with anti-mouse Alexa Fluor 488; C: representing the differential interference contrast (DIC); D: colocation of EhCRT with the C1q human protein (Channel Mergin). The micrographs showed the maximal projection of the -series. Scale bar represents 15  m.