BioMed Research International / 2014 / Article / Fig 6

Research Article

Entamoeba histolytica and E. dispar Calreticulin: Inhibition of Classical Complement Pathway and Differences in the Level of Expression in Amoebic Liver Abscess

Figure 6

Colocalization of EhCRT or EdCRT and human C1q after interaction with PMBC. C1q and EhCRT or EdCRT colocalization by confocal microscopy of (a) E. histolytica; (b) E. dispar trophozoites. Trophozoites were grown under axenic conditions and incubated during 30 min with PMBC; thereafter, C1q was added and incubated for 30 min. Then, the mixture was added with specific primary antibodies, anti-rabbit EhCRT IgG and mouse anti-human C1q IgG, respectively. The reaction was revealed with Alexa Fluor 555 goat anti-rabbit IgG and Alexa Fluor 488 goat anti-mouse IgG. The micrographs show the maximal projection of the -series. Scale bar represents 20  m. A: phase contrast microscopy; B: EhCRT (red); C: C1q (green); D: merge.