Entamoeba histolytica and E. dispar Calreticulin: Inhibition of Classical Complement Pathway and Differences in the Level of Expression in Amoebic Liver Abscess
Colocalization of EhCRT or EdCRT and human C1q after interaction with PMBC. C1q and EhCRT or EdCRT colocalization by confocal microscopy of (a) E. histolytica; (b) E. dispar trophozoites. Trophozoites were grown under axenic conditions and incubated during 30 min with PMBC; thereafter, C1q was added and incubated for 30 min. Then, the mixture was added with specific primary antibodies, anti-rabbit EhCRT IgG and mouse anti-human C1q IgG, respectively. The reaction was revealed with Alexa Fluor 555 goat anti-rabbit IgG and Alexa Fluor 488 goat anti-mouse IgG. The micrographs show the maximal projection of the -series. Scale bar represents 20 m. A: phase contrast microscopy; B: EhCRT (red); C: C1q (green); D: merge.