BioMed Research International / 2014 / Article / Fig 7

Research Article

Entamoeba histolytica and E. dispar Calreticulin: Inhibition of Classical Complement Pathway and Differences in the Level of Expression in Amoebic Liver Abscess

Figure 7

Colocalization of EhCRT or EdCRT and human C1q after interaction with NHS: human C1q and EhCRT or EdCRT colocalization estimated by confocal microscopy of (a) E. histolytica; (b) E. dispar trophozoites. Trophozoites were grown under axenic conditions and were allowed to adhere to sterile glass cover slips. Trophozoites were incubated with normal human serum (NHS) (source of C1q) and then fixed with 3.5% paraformaldehyde/PBS; thereafter, trophozoites were incubated with specific primary antibodies, rabbit anti-EhCRT IgG and mouse anti-human C1q IgG, respectively. The reaction was revealed with Alexa Fluor 555 goat anti-rabbit IgG and Alexa Fluor 488 goat anti-mouse IgG. The micrographs show the maximal projection of the -series. Scale bar represents 20  m. A: phase contrast microscopy; B: EhCRT (red); C: C1q (green); D: merge.