Research Article

Biodegradation Ability and Catabolic Genes of Petroleum-Degrading Sphingomonas koreensis Strain ASU-06 Isolated from Egyptian Oily Soil

Table 1

List of primers used for detection of mono- and dioxygenase genes.

PrimerSequence (5′ to 3′)Expected size (bp)Reference

alkBF
alkBR
5′-AACTACMTCGARCAYTACGG-3′ 
5′-TGAMGATGTGGTYRCTGTTCC-3′
100Powell et al. [22]
AlkB1F
AlkB1R
5′-TACGGGCACTTCGCGATTGA-3′ 
5′-CGCCCAGTTCGAMACGATGTG-3′
550Kloos et al. [13]
nahAc F
nahAc R
5′-TGGCGATGAAGAACTTTTCC-3′ 
5′-AACGTACGCTGAACCGAGTC-3′
487 Laurie and Jones [14]
PAH-RHD-GPf
PAH-RHGPr
5′-CGG CGC CGA CAA YTT YGT NGG-3′ 
5′-GGG GAA CAC GGT GCC RTG DAT RAA-3′
292Cébron et al. [12]
C12OF
C12OR
5′-GCCAACGTCGACGTCTGGCAGCA-3′ 
5′-CGCCTTCAAAGTTGATCTGCGTGGTTGGT-3′
350Sei et al. [23]
C23OF
C23OR
5′-AAGAGGCATGGGGGCGCACCGGTTCGA-3′ 
5′-TCACCAGCAAACACCTCGTTGCGGTTGCC-3′
900Sei et al. [23]