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BioMed Research International
Volume 2014, Article ID 128046, 9 pages
http://dx.doi.org/10.1155/2014/128046
Research Article

Endothelial Function in a Mouse Model of Myeloperoxidase Deficiency

1Department of Physiology, Institute of Neuroscience and Physiology, Sahlgrenska Academy, University of Gothenburg, P.O. Box 432, 405 30 Gothenburg, Sweden
2Department of Molecular Pharmacology, AstraZeneca R&D, Pepparedsleden 1, 431 83 Mölndal, Sweden

Received 30 September 2013; Revised 7 December 2013; Accepted 31 December 2013; Published 23 February 2014

Academic Editor: Vladimir V. Matchkov

Copyright © 2014 Veronika Golubinskaya et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Myeloperoxidase (MPO) activity is suggested to reduce the function of vascular nitric oxide, thereby contributing to endothelial dysfunction, although data in rodents are inconclusive. We examined vascular contractile and relaxant responses in MPO-deficient () and wild-type mice to investigate the role for myeloperoxidase in the development of endothelial dysfunction. Carotid and saphenous arteries were taken from 8-month-old mice and studied in a myograph. Responses of carotid arteries to phenylephrine, high potassium, or acetylcholine (Ach) were statistically not different from controls. Treatment with lipopolysaccharide (LPS; to enhance endothelial dysfunction) reduced responses to Ach in but did not affect responses in wild-type. In response to high concentrations of Ach, carotid arteries responded with transient contractions, which were not different between the groups and not affected by LPS treatment. Saphenous arteries from had smaller normalized diameters and developed less contractile force. Vessels from were less sensitive to Ach than controls. These data suggest that mature MPO-deficient mice do not show enhanced endothelial function compared to wild-type mice, even when provoked with LPS treatment. The EDHF response appears to be reduced in MPO deficiency.