Adipose Tissue-Derived Stem Cell Secreted IGF-1 Protects Myoblasts from the Negative Effect of Myostatin
Representative Western blot analysis of MyoD protein expression. Nontreated cells clearly showed an expression of MyoD protein (lane 2) whereas no MyoD protein was detected after incubation with myostatin (lane 3). After incubation with CM of ASCs a weak but significant expression of MyoD was detectable by Western blot analyses (lane 4). A total protein amount of 90 g was loaded on each lane. Green arrow indicates the band of MyoD and red arrow indicates -Actin as loading control ().