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BioMed Research International
Volume 2014, Article ID 135082, 9 pages
http://dx.doi.org/10.1155/2014/135082
Research Article

Phyllanthus wightianus Müll. Arg.: A Potential Source for Natural Antimicrobial Agents

1Natural Drug Research Laboratory, Department of Biotechnology, Periyar University, Periyar Palkalai Nagar, Salem, Tamil Nadu 636 011, India
2Molecular Entomology Laboratory, Department of Biotechnology, Periyar University, Periyar Palkalai Nagar, Salem, Tamil Nadu 636 011, India

Received 5 February 2014; Revised 9 April 2014; Accepted 9 April 2014; Published 5 May 2014

Academic Editor: Kota V. Ramana

Copyright © 2014 D. Natarajan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Phyllanthus wightianus belongs to Euphorbiaceae family having ethnobotanical importance. The present study deals with validating the antimicrobial potential of solvent leaf extracts of P. wightianus. 11 human bacterial pathogens (Bacillus subtilis, Streptococcus pneumoniae, Staphylococcus epidermidis, Proteus vulgaris, Pseudomonas aeruginosa, Klebsiella pneumoniae, Salmonella typhimurium, Escherichia coli, Shigella flexneri, Proteus vulgaris, and Serratia marcescens) and 4 fungal pathogens (Candida albicans, Cryptococcus neoformans, Mucor racemosus, and Aspergillus niger) were also challenged with solvent leaf extracts usingagar well and disc diffusion methods. Further, identification of the active component present in the bioactive extract was done using GC-MS analysis. Results show that all extracts exhibited broad spectrum (6–29 mm) of antibacterial activity on most of the tested organisms. The results highlight the fact that the well in agar method was more effective than disc diffusion method. Significant antimicrobial activity was detected in methanol extract against S. pneumoniae (29 mm) with MIC and MBC values of 15.62 μg/mL. GC-MS analysis revealed that 29 bioactive constituents were present in methanolic extract of P. wightianus, of which 9,12-octadecaenioic acid (peak area 22.82%; RT-23.97) and N-hexadecanoic acid (peak area 21.55% RT-21.796) are the major compounds. The findings of this study show that P. wightianus extracts may be used as an anti-infective agent in folklore medicine.