Negative Regulation of GADD34 on Myofibroblasts during Cutaneous Wound Healing
Figure 5
Induction of myofibroblast apoptosis by GADD34. (a) Immunostaining of the wound granulation tissues on day 8 was performed using an antibody against α-SMA (red), followed by TUNEL staining [27] and DAPI (blue). Scale bar: 100 μm. (b) The percentage of α-SMA+TUNEL+ double positive cells was calculated. Bar graph shown is mean ± SD. . (c) Immunoblot analyses were performed to assess the levels of cleaved caspase 3 in WT and GADD34−/− mice. Statistics shown represent 3 independent experiments.