Alleviation of Plasma Homocysteine Level by Phytoestrogen -Zearalanol Might Be Related to the Reduction of Cystathionine -Synthase Nitration

Figure 3

𝛼-Zearalanol treatment decreased 3-nitrotyrosine content in the livers of HHcy rats. The 3-nitrotyrosine content was detected by Western blot (normalized by GAPDH expression) (a) and immunohistochemistry (b). Data were expressed as mean ± SD; 𝑛=3. Student 𝑡-test; *𝑃<0.05 versus HHcy. HHcy: hyperhomocysteinemia; NT: 3-nitrotyrosine; 𝛼-ZAL: 𝛼-zearalanol.

(a)

(b)