Table 3: Distribution of  99mTc-lorazepam from LS (i.v.), LS (i.n.), and Lzp-PLGA-NPs (i.n.) at different time intervals in Sprague-Dawley rats*.

Formulation and route of administration Distribution of lorazepam in blood and brain compartments at different
Distribution sampling time points
Organ/tissue0.5 h1 h2 h4 h8 h

LS (i.v)Blood2.976 ± 0.12.737 ± 0.152.350 ± 0.31.813 ± 0.251.524 ± 0.15
Brain1.547 ± 0.21.512 ± 0.31.396 ± 0.320.826 ± 0.110.610 ± 0.12

LS (i.n)Blood1.404 ± 0.21.545 ± 0.21.210 ± 0.040.556 ± 0.160.359 ± 0.1
Brain0.673 ± 0.21.148 ± 0.250.923 ± 0.20.335 ± 0.080.247 ± 0.2

Lzp-PLGA-NPs (i.n)Blood1.769 ± 0.31.916 ± 0.182.062 ± 0.182.125 ± 0.351.507 ± 0.1
Brain1.399 ± 0.11.624 ± 0.231.794 ± 0.151.388 ± 0.220.104 ± 0.2

LS (i.v)Brain/blood0.519 ± 0.230.553 ± 0.250.594 ± 0.20.456 ± 0.050.400 ± 0.2

LS (i.n)Brain/blood0.479 ± 0.150.743 ± 0.10.763 ± 0.150.602 ± 0.10.687 ± 0.3

Lzp-PLGA-NPs (i.n)Brain/blood0.791 ± 0.20.847 ± 0.150.870 ± 0.10.653 ± 0.250.694 ± 2.5

The rats were administered 100  Ci  99mTc-lorazepam and the radioactivity was measured in percent per gram of tissue of the administered dose. Each value is the mean ± SD of three estimations. Radioactivity was measured at 0 h and all the measurements were performed using 0 h sample corresponding tissue/organ as blank sample.