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BioMed Research International
Volume 2014, Article ID 157216, 7 pages
http://dx.doi.org/10.1155/2014/157216
Research Article

The Effects of Apigenin on the Expression of Fas/FasL Apoptotic Pathway in Warm Liver Ischemia-Reperfusion Injury in Rats

1Second Department of Surgery, Medical School, Democritus University of Thrace, University Hospital of Alexandroupolis, 681 00 Alexandroupolis, Greece
2Laboratory of Experimental Surgery and Surgical Research, Medical School, Democritus University of Thrace, University Hospital of Alexandroupolis, 681 00 Alexandroupolis, Greece
3Department of Pharmacology, Medical School, Democritus University of Thrace, University Hospital of Alexandroupolis, 681 00 Alexandroupolis, Greece
4Laboratory of Histology, Medical School, Democritus University of Thrace, University Hospital of Alexandroupolis, 681 00 Alexandroupolis, Greece
5Laboratory of Medical Statistics, Medical School, Democritus University of Thrace, University Hospital of Alexandroupolis, 681 00 Alexandroupolis, Greece

Received 19 February 2014; Revised 15 June 2014; Accepted 18 June 2014; Published 6 July 2014

Academic Editor: Senthil K. Venugopal

Copyright © 2014 Evanthia G. Tsalkidou et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background. The aim of this experimental study was to investigate the role of apigenin in liver apoptosis, in an experimental model of hepatic ischemia-reperfusion in rats. Materials and Methods. Forty-eight Wistar rats (apigenin and control groups), 14 to 16 weeks old and weighing 220 to 350 g, were used. They were all subjected to hepatic ischemia by occlusion of the hepatic artery and portal vein for 45 minutes and reperfusion was followed for 60, 120, and 240 minutes. Apigenin was administrated intraperitoneally. Liver tissues were used for the detection of apoptosis by TUNEL assay and caspase 3 antibodies. Expression analysis of Fas/FasL genes was evaluated by real time PCR. Results. The expression analysis of Fas and FasL genes was increasing during reperfusion (significantly in the group of 240 minutes of reperfusion). It was in the same group that apigenin decreased Fas receptor levels and inhibited apoptosis as confirmed by TUNEL assay and caspase 3 antibodies. Conclusions. The effects of apigenin in the Fas/FasL mediated pathway of apoptosis, in the hepatic ischemia-reperfusion, seem to have a protective result on the hepatic cell.