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BioMed Research International
Volume 2014, Article ID 157987, 9 pages
Research Article

PHBV/PAM Scaffolds with Local Oriented Structure through UV Polymerization for Tissue Engineering

1Department of Biomedical Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, China
2Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Jinan University, Guangzhou 510632, China
3Biomaterial Research Institute, School of Materials Science and Engineering, South China University of Technology, Guangzhou 510641, China
4National Engineering Research Center for Tissue Restoration and Reconstruction, Guangzhou 510006, China

Received 28 April 2013; Accepted 21 October 2013; Published 22 January 2014

Academic Editor: Alejandro Comellas

Copyright © 2014 Yu Ke et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Locally oriented tissue engineering scaffolds can provoke cellular orientation and direct cell spread and migration, offering an exciting potential way for the regeneration of the complex tissue. Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) scaffolds with locally oriented hydrophilic polyacrylamide (PAM) inside the macropores of the scaffolds were achieved through UV graft polymerization. The interpenetrating PAM chains enabled good interconnectivity of PHBV/PAM scaffolds that presented a lower porosity and minor diameter of pores than PHBV scaffolds. The pores with diameter below 100 μm increased to 82.15% of PHBV/PAM scaffolds compared with 31.5% of PHBV scaffolds. PHBV/PAM scaffold showed a much higher compressive elastic modulus than PHBV scaffold due to PAM stuffing. At 5 days of culturing, sheep chondrocytes spread along the similar direction in the macropores of PHBV/PAM scaffolds. The locally oriented PAM chains might guide the attachment and spreading of chondrocytes and direct the formation of microfilaments via contact guidance.