The Role of Ubiquitination and Sumoylation in Diabetic Nephropathy
Figure 4
The result of our experiments. (a) SUMO1 and SUMO2/3 protein expression after various glucose concentrations’ challenge determined by Western blot. (b) IκBα protein expression after various glucose concentrations’ challenge determined by Western blot. (c) NF-κBp65 protein expression after various glucose concentrations’ challenge determined by Western blot. (d) MCP-1 mRNA expression after various glucose concentrations’ challenge determined by RT-PCR. The expression of SUMO1 and SUMO2/3 enhanced in rat glomerular mesangial cells induced by high glucose, while IκBα sumoylation decreased significantly, but NF-κBp65 and MCP-1 were increased under high glucose conditions.
