Lipid Nanoparticles as Carriers for RNAi against Viral Infections: Current Status and Future Perspectives

Table 3

Studies carried out with lipid-based nanosystems as RNAi delivery vectors against hepatitis B (HBV).

Lipid nanosystem	RNAi	Targeting molecule	Culture cells	In vivo model	Reference
SNALP	HBV siRNAs chemically stabilized for nuclease resistance	—	HBV-replicating HepG2	HBV mouse model constructed by hydrodynamic injection of HBV vector DNA	[47]
Cationic liposomes	HBV-X specific siRNA (siHBV)	Apo A-I	HepG2 and Huh7	Acute HBV-infected mouse model by hydrodynamic injection of a plasmid	[48]
PEGylated cationic liposomes	HBV specific siRNA	—	Huh7 cells previously transfected with HBV replication target plasmid	HBV transgenic mice	[49]
DODAG 8 lipid	HBV specific siRNA	—	—	HBV transgenic mice	[50]
Cationic liposomes	Altriol modified HBV-X siRNA	Galactose	Huh7 cells previously transfected with HBV target DNA plasmid	HBV transgenic mice	[51]

SNALP: stable nucleic acid lipid particle; HepG2: liver hepatocellular carcinoma cells; HuH7: human hepatoma cell line; DODAG: , -dioctadecyl-N-4,8- diaza-10-aminodecanoylglycine amide.