BioMed Research International / 2014 / Article / Fig 5

Research Article

Trichostatin A Suppresses EGFR Expression through Induction of MicroRNA-7 in an HDAC-Independent Manner in Lapatinib-Treated Cells

Figure 5

TSA suppressed the 3′UTR activity of EGFR mRNA. MDA-MB-231 and 231/Lap#6 cells were cotransfected with EGFR 3′UTR-luciferase plasmid and β-galactosidase for 24 hrs followed by treatment with 1 μM TSA or SAHA for another 24 hrs. Total lysates were then prepared and subjected to luciferase and β-galactosidase activity assays. The relative luciferase activity was normalized to β-galactosidase.