Table of Contents Author Guidelines Submit a Manuscript
BioMed Research International
Volume 2014, Article ID 171548, 8 pages
http://dx.doi.org/10.1155/2014/171548
Research Article

Phenotypic and Molecular Characterization of Plasmid Mediated AmpC β-Lactamases among Escherichia coli, Klebsiella spp., and Proteus mirabilis Isolated from Urinary Tract Infections in Egyptian Hospitals

1Department of Microbiology & Immunology, Faculty of Medicine, Zagazig University, Zagazig, Sharqia,, Egypt
2Department of Microbiology and Immunology, Faculty of Pharmacy, October University for Modern Sciences and Arts (MSA), 26 July Road Intersection with El Wahat Road, 6th of October, Giza, Egypt

Received 14 February 2014; Revised 16 May 2014; Accepted 18 May 2014; Published 9 June 2014

Academic Editor: Frederick D. Quinn

Copyright © 2014 Mai M. Helmy and Reham Wasfi. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. A. Bauernfeind, Y. Chong, and S. Schweighart, “Extended broad spectrum β-lactamase in Klebsiella pneumoniae including resistance to cephamycins,” Infection, vol. 17, no. 5, pp. 316–321, 1989. View at Google Scholar · View at Scopus
  2. G. A. Jacoby, “AmpC Β-lactamases,” Clinical Microbiology Reviews, vol. 22, no. 1, pp. 161–182, 2009. View at Publisher · View at Google Scholar · View at Scopus
  3. S. Singhal, T. Mathur, S. Khan et al., “Evaluation of methods for AmpC β-lactamase in gram negative clinical isolates from tertiary care hospitals,” Indian Journal of Medical Microbiology, vol. 23, no. 2, pp. 120–124, 2005. View at Google Scholar · View at Scopus
  4. D. M. Livermore, “Current epidemiology and growing resistance of Gram-negative pathogens,” Korean Journal of Internal Medicine, vol. 27, no. 2, pp. 128–142, 2012. View at Publisher · View at Google Scholar · View at Scopus
  5. D. Maina, G. Revathi, S. Kariuki, and H. Ozwara, “Genotypes and cephalosporin susceptibility in extended-spectrum beta-lactamase producing Enterobacteriaceae in the community,” Journal of Infection in Developing Countries, vol. 6, no. 6, pp. 470–477, 2012. View at Google Scholar · View at Scopus
  6. K. H. Rand, B. Turner, H. Seifert, C. Hansen, J. A. Johnson, and A. Zimmer, “Clinical laboratory detection of AmpC β-lactamase: does it affect patient outcome?” The American Journal of Clinical Pathology, vol. 135, no. 4, pp. 572–576, 2011. View at Publisher · View at Google Scholar · View at Scopus
  7. Clinical and Laboratory Standards Institute CLSI, Performance standards for antimicrobial susceptibility testing, 24 th informational supplement M100-S24, 2014.
  8. E. S. Moland, J. A. Black, J. Ourada, M. D. Reisbig, N. D. Hanson, and K. S. Thomson, “Occurrence of newer β-lactamases in Klebsiella pneumoniae isolates from 24 U.S. Hospitals,” Antimicrobial Agents and Chemotherapy, vol. 46, no. 12, pp. 3837–3842, 2002. View at Publisher · View at Google Scholar · View at Scopus
  9. Y. Doi and D. L. Paterson, “Detection of plasmid-mediated class C β-lactamases,” International Journal of Infectious Diseases, vol. 11, no. 3, pp. 191–197, 2007. View at Publisher · View at Google Scholar · View at Scopus
  10. D. Yong, R. Park, J. H. Yum, K. Lee, E. C. Choi, and Y. Chong, “Further modification of the Hodge test to screen AmpC β-lactamase (CMY-1)-producing strains of Escherichia coli and Klebsiella pneumoniae,” Journal of Microbiological Methods, vol. 51, no. 3, pp. 407–410, 2002. View at Publisher · View at Google Scholar · View at Scopus
  11. J. A. Black, E. S. Moland, and K. S. Thomson, “AmpC disk test for detection of plasmid-mediated AmpC β-lactamases in Enterobacteriaceae lacking chromosomal AmpC β-lactamases,” Journal of Clinical Microbiology, vol. 43, no. 7, pp. 3110–3113, 2005. View at Publisher · View at Google Scholar · View at Scopus
  12. Y. T. Thean, L. S. Y. Ng, J. He, H. K. Tse, and Y. H. Li, “Evaluation of screening methods to detect plasmid-mediated AmpC in Escherichia coli, Klebsiella pneumoniae, and Proteus mirabilis,” Antimicrobial Agents and Chemotherapy, vol. 53, no. 1, pp. 146–149, 2009. View at Publisher · View at Google Scholar · View at Scopus
  13. N. P. Brenwald, G. Jevons, J. Andrews, L. Ang, and A. P. Fraise, “Disc methods for detecting AmpC β-lactamase-producing clinical isolates of Escherichia coli and Klebsiella pneumoniae,” Journal of Antimicrobial Chemotherapy, vol. 56, no. 3, pp. 600–601, 2005. View at Publisher · View at Google Scholar · View at Scopus
  14. D. M. Livermore, M. Warner, and S. Mushtaq, “Evaluation of the chromogenic Cica-β-Test for detecting extended-spectrum, AmpC and metallo-β-lactamases,” Journal of Antimicrobial Chemotherapy, vol. 60, no. 6, pp. 1375–1379, 2007. View at Publisher · View at Google Scholar · View at Scopus
  15. T. Yagi, J.-I. Wachino, H. Kurokawa et al., “Practical methods using boronic acid compounds for identification of class C β-lactamase-producing Klebsiella pneumoniae and Escherichia coli,” Journal of Clinical Microbiology, vol. 43, no. 6, pp. 2551–2558, 2005. View at Publisher · View at Google Scholar · View at Scopus
  16. F. J. Pérez-Pérez and N. D. Hanson, “Detection of plasmid mediated AmpC β-lactamase genes in clinical isolates by using multiplex PCR,” Journal of Clinical Microbiology, vol. 40, pp. 2153–2162, 2002. View at Google Scholar
  17. N. Fam, D. Gamal, M. El Said et al., “Prevalence of plasmid-mediated ampC genes in clinical isolates of Enterobacteriaceae from Cairo, Egypt,” British Microbiology Research Journal, vol. 3, no. 4, pp. 525–537, 2013. View at Google Scholar
  18. P. R. Murray, E. J. Baron, J. H. Jorgensen, M. L. Landry, and M. A. Pfaller, Manual of Clinical Microbiology, American Society for Microbiology, Washington, DC, USA, 9th edition, 2007.
  19. Clinical and Laboratory Standards Institute CLSI, Performance Standards for Antimicrobial Susceptibility Testing, Twenty First Informational Supplement, vol. 31, supplement 1, 2011, edited by: Franklin, R. and Matthew, A.
  20. G. Dalela, S. Gupta, D. K. Jain, and P. Mehta, “Antibiotic resistance pattern in uropathogens at a Tertiary Care Hospital at Jhalawar with special reference to ESβL, AmpC β-Lactamase and MRSA production,” Journal of Clinical and Diagnostic Research, vol. 6, no. 4, pp. 645–651, 2012. View at Google Scholar
  21. V. Lorian, Antibiotics in Laboratory Medicine, Lippincott Williams and Wilkins, Philadelphia, Pa, USA, 5th edition, 2005.
  22. P. E. Coudron, N. D. Hanson, and M. W. Climo, “Occurrence of extended-spectrum and ampC beta-lactamases in bloodstream isolates of Klebsiella pneumoniae: isolates harbor plasmid-mediated FOX-5 and ACT-1 AmpC beta-lactamases,” Journal of Clinical Microbiology, vol. 41, no. 2, pp. 772–777, 2003. View at Publisher · View at Google Scholar · View at Scopus
  23. V. Manchanda and N. P. Singh, “Occurrence and detection of AmpC β-lactamases among Gram-negative clinical isolates using a modified tree-dimensional test at Guru Tegh Bahadur hospital, Delhi, India,” Journal of Antimicrobial Chemotherapy, vol. 51, no. 2, pp. 415–418, 2003. View at Publisher · View at Google Scholar · View at Scopus
  24. W. Lee, B. Jung, S. G. Hong et al., “Comparison of 3 phenotypic-detection methods for identifying plasmid-mediated AmpC β-lactamase-producing Escherichia coli, Klebsiella pneumoniae, and Proteus mirabilis strains,” Korean Journal of Laboratory Medicine, vol. 29, no. 5, pp. 448–454, 2009. View at Publisher · View at Google Scholar · View at Scopus
  25. N. Fam, D. Gamal, M. El Said et al., “Detection of plasmid-mediated AmpC beta-lactamases in clinically significant bacterial isolates in a research institute hospital in Egypt,” Life Science Journal, vol. 10, no. 2, pp. 2294–2304, 2013. View at Google Scholar · View at Scopus
  26. N. O. Yilmaz, N. Agus, E. Bozcal, O. Oner, and A. Uzel, “Detection of plasmid-mediated AmpC β-lactamase in Escherichia coli and Klebsiella pneumoniae,” Indian Journal of Medical Microbiology, vol. 31, no. 1, pp. 53–59, 2013. View at Publisher · View at Google Scholar · View at Scopus
  27. V. Rawat, M. Singhai, A. Kumar, P. K. Jha, and R. Goyal, “Bacteriological and resistance profile in isolates from diabetic patients,” North American Journal of Medical Sciences, vol. 4, no. 11, pp. 563–568, 2012. View at Publisher · View at Google Scholar · View at Scopus
  28. M. R. Mulvey, E. Bryce, D. A. Boyd et al., “Molecular characterization of cefoxitin-resistant Escherichia coli from Canadian hospitals,” Antimicrobial Agents and Chemotherapy, vol. 49, no. 1, pp. 358–365, 2005. View at Publisher · View at Google Scholar · View at Scopus
  29. J.-M. Pages, J.-P. Lavigne, V. Leflon-Guibout et al., “Efflux pump, the masked side of ß-lactam resistance in Klebsiella pneumoniae clinical isolates,” PLoS ONE, vol. 4, no. 3, Article ID e4817, 2009. View at Publisher · View at Google Scholar · View at Scopus
  30. N. N. El-Hefnawy, Phenotypic detection of AmpC beta-lactamase in Gram-negative bacteria [M.S. thesis], Clinical and Chemistry Pathology, Faculty of Medicine, Ain Shams University, 2008.
  31. A. El Kholy, H. Baseem, G. S. Hall, G. W. Procop, and D. L. Longworth, “Antimicrobial resistance in Cairo, Egypt 1999-2000: a survey of five hospitals,” Journal of Antimicrobial Chemotherapy, vol. 51, no. 3, pp. 625–630, 2003. View at Publisher · View at Google Scholar · View at Scopus
  32. Y. S. Park, S. Yoo, M.-R. Seo, J. Y. Kim, Y. K. Cho, and H. Pai, “Risk factors and clinical features of infections caused by plasmid-mediated AmpC β-lactamase-producing Enterobacteriaceae,” International Journal of Antimicrobial Agents, vol. 34, no. 1, pp. 38–43, 2009. View at Publisher · View at Google Scholar · View at Scopus
  33. J. D. D. Pitout, P. G. Le, K. L. Moore, D. L. Church, and D. B. Gregson, “Detection of AmpC β-lactamases in Escherichia coli, Klebsiella spp., Salmonella spp. and Proteus mirabilis in a regional clinical microbiology laboratory,” Clinical Microbiology and Infection, vol. 16, no. 2, pp. 165–170, 2010. View at Publisher · View at Google Scholar · View at Scopus
  34. K. S. Thomson, “Extended-spectrum-β-lactamase, AmpC, and carbapenemase issues,” Journal of Clinical Microbiology, vol. 48, no. 4, pp. 1019–1025, 2010. View at Publisher · View at Google Scholar · View at Scopus
  35. P. E. Coudron, “Inhibitor-based methods for detection of plasmid-mediated AmpC β-lactamases in Klebsiella spp., Escherichia coli, and Proteus mirabilis,” Journal of Clinical Microbiology, vol. 43, no. 8, pp. 4163–4167, 2005. View at Publisher · View at Google Scholar · View at Scopus
  36. D. Nadjar, M. Rouveau, C. Verdet et al., “Outbreak of Klebsiella pneumoniae producing transferable AmpC-type β-lactamase (ACC-1) originating from Hafnia alvei,” FEMS Microbiology Letters, vol. 187, no. 1, pp. 35–40, 2000. View at Publisher · View at Google Scholar · View at Scopus
  37. E. Ruppé, P. Bidet, C. Verdet, G. Arlet, and E. Bingen, “First detection of the Ambler class C 1 AmpC β-lactamase in Citrobacter freundii by a new, simple double-disk synergy test,” Journal of Clinical Microbiology, vol. 44, no. 11, pp. 4204–4207, 2006. View at Publisher · View at Google Scholar · View at Scopus
  38. J. Empel, A. Baraniak, E. Literacka et al., “Molecular survey of β-lactamases conferring resistance to newer β-lactams in Enterobacteriaceae isolates from Polish Hospitals,” Antimicrobial Agents and Chemotherapy, vol. 52, no. 7, pp. 2449–2454, 2008. View at Publisher · View at Google Scholar · View at Scopus
  39. H. Adler, L. Fenner, P. Walter et al., “Plasmid-mediated AmpC β-lactamases in Enterobacteriaceae lacking inducible chromosomal ampC genes: prevalence at a Swiss university hospital and occurrence of the different molecular types in Switzerland,” Journal of Antimicrobial Chemotherapy, vol. 61, no. 2, pp. 457–458, 2008. View at Publisher · View at Google Scholar · View at Scopus
  40. A. E.-D. M. S. Hosny and M. T. Kashif, “A study on occurrence of plasmid mediated AmpC ß-lactamases among gram negative clinical isolates and evaluation of different methods used for their detection,” Journal of Applied Sciences Research, vol. 8, no. 4, pp. 2280–2285, 2012. View at Google Scholar · View at Scopus
  41. R. Barwa, E. Abdelmegeed, and K. Abd El Galil, “Occurrence and detection of AmpC β-lactamases among some clinical isolates of Enterobacteriaceae obtained from Mansoura University Hospitals, Egypt,” African Journal of Microbiology Research, vol. 6, no. 41, pp. 6924–6930, 2012. View at Google Scholar
  42. M. Wassef, I. M. Behiry, M. Younan, N. El Guindy, S. Mostafa, and E. Abada, “Genotypic identification of AmpC β-lactamases production in gram-negative Bacilli isolates,” Jundishapur Journal of Microbiology, vol. 7, no. 1, Article ID e8856, 2014. View at Google Scholar
  43. A. Manoharan, M. Sugumar, A. Kumar, H. Jose, and D. Mathai, “Phenotypic & molecular characterization of AmpC β-lactamases among Escherichia coli, Klebsiella spp. & Enterobacter spp. from five Indian Medical Centers,” Indian Journal of Medical Research, vol. 135, no. 3, pp. 359–364, 2012. View at Google Scholar · View at Scopus
  44. R. C. da Silva Dias, A. A. Borges-Neto, G. I. D'Almeida Ferraiuoli, M. P. de-Oliveira, L. W. Riley, and B. M. Moreira, “Prevalence of AmpC and other β-lactamases in enterobacteria at a large urban university hospital in Brazil,” Diagnostic Microbiology and Infectious Disease, vol. 60, no. 1, pp. 79–87, 2008. View at Publisher · View at Google Scholar · View at Scopus
  45. N. Woodford, S. Reddy, E. J. Fagan et al., “Wide geographic spread of diverse acquired AmpC β-lactamases among Escherichia coli and Klebsiella spp. in the UK and Ireland,” Journal of Antimicrobial Chemotherapy, vol. 59, no. 1, pp. 102–105, 2007. View at Publisher · View at Google Scholar · View at Scopus
  46. B. Haldorsen, B. Aasnaes, K. H. Dahl et al., “The AmpC phenotype in Norwegian clinical isolates of Escherichia coli is associated with an acquired IS Ecp1-like ampC element or hyperproduction of the endogenous AmpC,” Journal of Antimicrobial Chemotherapy, vol. 62, no. 4, pp. 694–702, 2008. View at Publisher · View at Google Scholar · View at Scopus
  47. C. H. Chiu, L. H. Su, C. Chu et al., “Isolation of Salmonella enterica serotype choleraesuis resistant to ceftriaxone and ciprofloxacin,” The Lancet, vol. 363, no. 9417, pp. 1285–1286, 2004. View at Publisher · View at Google Scholar · View at Scopus
  48. L.-H. Su, H.-L. Chen, J.-H. Chia et al., “Distribution of a transposon-like element carrying blaCMY-2 among Salmonella and other Enterobacteriaceae,” Journal of Antimicrobial Chemotherapy, vol. 57, no. 3, pp. 424–429, 2006. View at Publisher · View at Google Scholar · View at Scopus
  49. L. Armand-Lefèvre, V. Leflon-Guibout, J. Bredin et al., “Imipenem resistance in Salmonella enterica serovar Wien related to porin loss and CMY-4 β-lactamase production,” Antimicrobial Agents and Chemotherapy, vol. 47, no. 3, pp. 1165–1168, 2003. View at Publisher · View at Google Scholar · View at Scopus
  50. S. Dahmen, W. Mansour, K. Charfi, N. Boujaafar, G. Arlet, and O. Bouallègue, “Imipenem resistance in Klebsiella pneumoniae is associated to the combination of plasmid-mediated CMY-4 AmpC β-Lactamase and loss of an outer membrane protein,” Microbial Drug Resistance, vol. 18, no. 5, pp. 479–483, 2012. View at Publisher · View at Google Scholar · View at Scopus
  51. C. Mata, E. Miró, A. Rivera, B. Mirelis, P. Coll, and F. Navarro, “Prevalence of acquired AmpCβ-lactamases in Enterobacteriaceae lacking inducible chromosomal ampC genes at a Spanish hospital from 1999 to 2007,” Clinical Microbiology and Infection, vol. 16, no. 5, pp. 472–476, 2010. View at Publisher · View at Google Scholar · View at Scopus
  52. C. Verdet, G. Arlet, G. Barnaud, P. H. Lagrange, and A. Philippon, “A novel integron in Salmonella enterica serovar enteritidis, carrying the bla(DHA-1) gene and its regulator gene ampR, originated from Morganella morganii,” Antimicrobial Agents and Chemotherapy, vol. 44, no. 1, pp. 222–225, 2000. View at Google Scholar · View at Scopus
  53. H. Pai, C.-I. Kang, J.-H. Byeon et al., “Epidemiology and clinical features of bloodstream infections caused by AmpC-type-β-lactamase-producing Klebsiella pneumoniae,” Antimicrobial Agents and Chemotherapy, vol. 48, no. 10, pp. 3720–3728, 2004. View at Publisher · View at Google Scholar · View at Scopus
  54. E. S. Moland, S. Y. Kim, S. G. Hong, and K. S. Thomson, “Newer beta-lactamases: clinical and laboratory implications, part I,” Clinical Microbiology Newsletter, vol. 30, pp. 71–77, 2008. View at Google Scholar