p53 Is a Key Regulator for Osthole-Triggered Cancer Pathogenesis
Figure 4
JNK activation contributes to osthole-induced p53 activation in SW480 human colon cancer. (a) Cells were incubated with various concentrations of osthole for 24 h (left panel). Cells were transfected with DN-JNK or empty vector for 24 h (right panel) before cell lysates were collected. Levels of phosphorylated JNK were determined by western blot. (b) Cells were pretreated with or without SP600125 (10 μM) followed by stimulation with osthole for another 24 h; the protein expressions of p53, p-p53, and acetyl-p53 were examined by western blot analysis. Results are the representative of three independent experiments. (c) Cells were transfected with DN-JNK for 24 h followed by stimulation with osthole for another 24 h, and cell viability was examined by MTT (c) or SRB (d) assays. Osthole-inhibited migratory activities were examined by cell culture insert system after transfecting cells with DN-JNK or empty vector for 24 h (e). (f) Cells were pretreated with SP600125 (10 μM) followed by stimulation with osthole for another 24 h, and migratory activities were also examined.
