Interaction Analysis through Proteomic Phage Display

Table 1

Summary of high-throughput methods for identification of PPIs, types of interactions identified, and major advantages and disadvantages of the respective method.

Method	Type of interaction	Advantage	Disadvantage
AP-MS	Binary and; complexes	Physiological	Bias towards stable interactions,; limited to specific condition (e.g., cell type)
LUMIER	Binary and; complexes	Physiological	Bias towards stable interactions
Y2H	Binary	Low-tech	Bias towards stable interactions,; bias towards soluble proteins that can translocate to the nucleus
Combinatorial peptide phage display	Binary	Large library size (up to 10^10); Identification of consensus motifs	Need for bioinformatics,; limited to natural amino acids,; limited to protein-peptide interactions
Proteomic phage display	Binary	Identification of target proteins and consensus motif	Limited to natural amino acids