Glioprotective Effects of Ashwagandha Leaf Extract against Lead Induced Toxicity
(a) Immunostaining of HSP70 in control (C), ASH-WEX (0.1%) pretreatment (CA), lead nitrate 200 μM (LN), and lead nitrate after pretreatment with ASH-WEX (LN + AS) treated C6 cells. (b) Histogram depicts staining intensity measurement of HSP70 immunofluorescence indicating HSP70 expression levels in the cells from different treatment groups. (c) Representative Western blots and relative expression levels as analysed by densitometry and normalized against α-tubulin expression levels. The data represents mean ± SEM from three independent experiments. (d) Representative RT-PCR products of HSP70 and β-actin (internal control) mRNA in different treatment groups. Value of was considered to be significant. “” represents significant difference in comparison to control; “#” represents significant difference between LN and LN + AS groups.
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