BioMed Research International / 2014 / Article / Fig 4

Research Article

Glioprotective Effects of Ashwagandha Leaf Extract against Lead Induced Toxicity

Figure 4

ASH-WEX pretreatment normalizes lead induced changes in mortalin expression levels. (a) Immunostaining of mortalin in C6 cells from control (C), ASH-WEX (0.1%) pretreatment (CA), lead nitrate 200 μM (LN), and lead nitrate after pretreatment with ASH-WEX (LN + AS) treated groups. (b) Histogram shows expression levels of mortalin as analysed by intensity measurement using Image-Pro Plus software. (c) Representative Western blot of mortalin expression and histogram depicts relative expression levels of mortalin normalized against α-tubulin in C, CA, LN, and LN + AS groups as analysed by densitometry. Data are calculated from three independent experiments and represented as the mean ± SEM. (d) Representative RT-PCR products of mortalin and β-actin mRNA in all the groups under study. Mortalin expression levels were significantly lowered down by ASH-WEX pretreatment in LN + AS group as compared to LN group as shown by immunostaining, Western blotting, and RT-PCR. Value of was considered to be significant. “ ” represents significant difference in comparison to control; “#” represents significant difference between LN and LN + AS groups.

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