Glioprotective Effects of Ashwagandha Leaf Extract against Lead Induced Toxicity
(a) Representative immunofluorescent images of 30 μm thick coronal sections showing GFAP expression in dentate gyrus region of hippocampus, median eminence region of hypothalamus, and piriform cortex in vehicle control (C), lead nitrate (LN), and simultaneous lead nitrate and ASH-WEX (LN + AS) treated animal brains ( = 4-5). A marked increase in expression of GFAP was observed in the animals treated with lead nitrate as compared to control. The lead nitrate and ASH-WEX treated group showed normalization in expression of GFAP levels. Relative expression levels of GFAP were analysed using intensity measurement command of Image-Pro Plus software in hippocampus (b), hypothalamus (c), and cortex (d) shown as histograms. (e) Representative Western blot for GFAP and α-tubulin expression levels for different brain regions—hippocampus, hypothalamus, and cortex from C, LN, and LN + AS treated animals. GFAP protein expression levels were normalized against α-tubulin and data was plotted as histograms. Bar shows the mean ± SEM values. (f) depicts specific immunostaining for GFAP shown above as no specific signal was visible in the secondary antibody control group. was considered significant. “” represents significant difference between C and other groups and “#” represents significant difference between LN and LN + AS groups.
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