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BioMed Research International
Volume 2014, Article ID 196712, 7 pages
http://dx.doi.org/10.1155/2014/196712
Research Article

In Vitro Inhibition of Hepatitis C Virus by Antisense Oligonucleotides in PBMC Compared to Hepatoma Cells

1Microbial Biotechnology Department, National Research Center, Cairo 12311, Egypt
2Reproductive Health and Family Planning Department, National Research Center, Cairo, Egypt
3INRS-Institut Armand Frappier, Laval, QC, Canada H7V 1B7
4Chemistry Department, Faculty of Science, Cairo University, Cairo 12613, Egypt

Received 17 February 2014; Revised 7 April 2014; Accepted 10 April 2014; Published 1 June 2014

Academic Editor: Ramesh Prabhu

Copyright © 2014 Samar Samir Youssef et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Aim. To assess the efficiency of phosphorothioate antisense oligodeoxynucleotide 1 (S-ODN1) on HCV translation inhibition in PBMC compared to hepatoma cells in vitro for the first time. Materials and Methods. The study included 34 treatment naive HCV patients. IRES domain III and IV sequence variations were tested in 45 clones from 9 HCV patients. PBMC of HCV positive patients were subjected to S-ODN in vitro. Concomitantly HepG2 cells infected by the same patient’s serum were also treated with S-ODN1 for 24 and 48 hours. Cellular RNA was tested for HCV plus and minus strands by reverse transcription polymerase chain reaction (RT-PCR). Results. Sequence variations were seen in HCV IRES domain III only while domain IV was conserved among all the tested patient’s clones. S-ODN1 successfully inhibited HCV translation in HepG2 cells, while in PBMC inhibition was partial. Conclusion. HCV IRES domain IV is more conserved than domain IIId in genotype 4 HCV patients. S-ODN against HCV IRES domain IV was not efficient to inhibit HCV translation in PBMC under the study conditions. Further studies testing other S-ODN targeting other HCV IRES domains in PBMC should be done.