Reliable Assessment and Quantification of the Fluorescence-Labeled Antisense Oligonucleotides In Vivo
Figure 3
(a) Schematic representation of the experimental procedure. (b) Ratio of the total emission from liver and kidneys (, = 1). (c) In vivo organ imaging using confocal microscope. Scale 100 μm. ML = median lobe, RL = right lateral and caudal lobe, and LL = left lateral lobe. The organs were obtained from mice at 3 hours after injection via tail vein. (d) Area fraction of antisense-Alexa 594 in mice sacrificed after 3 hours.
