Validation of Reference Genes for Normalization Gene Expression in Reverse Transcription Quantitative PCR in Human Normal Thyroid and Goiter Tissue

Table 2

Primers sequence, product length, and NCBI reference sequence for reference genes by RT-qPCR.

Gene symbol	Name	Forward and reverse primers	Product length (bp)	NCBI reference sequence
ACTB	Beta-actin	5′-CTGGAACGGTGAAGGTGACA-3′	140	NM_001101.3
		5′-AAGGGACTTCCTGTAACAATGCA-3′
B2M	Beta-2-microglobulin	5′-CTATCCAGCGTACTCCAAAG-3′	165	NM_004048.2
		5′-ACAAGTCTGAATGCTCCACT-3′
GAPDH	Glyceraldehyde-3-phosphate dehydrogenase	5′-CTTTGTCAAGCTCATTTCCTGG-3′	133	NM_002046.3
		5′-TCTTCCTCTTGTGCTCTTGC-3′
HPRT1	Hypoxanthine phosphoribosyltransferase 1	5′-AGATGGTCAAGGTCGCAAG-3′	128	NM_000194.2
		5′-GTATTCATTATAGTCAAGGGCATATCC-3′
SDHA	Succinate dehydrogenase complex, subunit A, flavoprotein (Fp)	5′-TGGTTGTCTTTGGTCGGG-3′	85	NM_004168.2
		5′-GCGTTTGGTTTAATTGGAGGG-3′
YWHAZ	Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide	5′-CAACACATCCTATCAGACTGGG-3′; 5′-AATGTATCAAGTTCAGCAATGGC-3′	133	NM_001135699.1

bp: base pair. Modified from Souza et al., 2012 [17].