IL-6 Impairs Myogenic Differentiation by Downmodulation of p90RSK/eEF2 and mTOR/p70S6K Axes, without Affecting AKT Activity

Figure 3

Exogenous IL-6 impairs the expression of myogenin, without affecting the expression of Pax-7 and MyoD. C2C12 myoblasts were cultured in GM until subconfluence and then shifted to DM or DM containing 25 ng/mL of recombinant IL-6. After 48 h in the differentiation media, cells were harvested, total RNA was extracted, and first-strand cDNA was generated from 1 μg of total RNA to perform real-time PCR using specific Taqman probes. Expression data were normalized using actin beta (ACTB) as housekeeping gene. Results are expressed as means ± standard deviations. ^∗∗𝑃<0.005; n.s., not significant (Student’s 𝑡-test).

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