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BioMed Research International
Volume 2014, Article ID 209548, 7 pages
Research Article

A Novel Platelet Concentrate: Titanium-Prepared Platelet-Rich Fibrin

1Department of Periodontology, Haydarpasa Training Hospital, Gulhane Military Medical Academy, Üsküdar, 34618 Istanbul, Turkey
2Department of Periodontology, School of Dentistry, Cumhuriyet University, 58140 Sivas, Turkey
3Department of Medical Pathology, Gulhane Military Medical Academy, 34618 Istanbul, Turkey
4Department of Prosthodontics, School of Dentistry, Selcuk University, 42250 Konya, Turkey
5Department of Periodontology, School of Dentistry, Kocaeli University, 41380 Izmit, Turkey
6Department of Periodontology, School of Dentistry, Istanbul University, 34303 Istanbul, Turkey

Received 24 April 2013; Accepted 2 November 2013; Published 21 January 2014

Academic Editor: Fausto Catena

Copyright © 2014 Mustafa Tunalı et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


We developed a new product called titanium-prepared platelet-rich fibrin (T-PRF). The T-PRF method is based on the hypothesis that titanium may be more effective in activating platelets than the silica activators used with glass tubes in Chouckroun’s leukocyte- and platelet-rich fibrin (L-PRF) method. In this study, we aimed to define the structural characteristics of T-PRF and compare it with L-PRF. Blood samples were collected from 10 healthy male volunteers. The blood samples were drawn using a syringe. Nine milliliters was transferred to a dry glass tube, and 9 mL was transferred to a titanium tube. Half of each clot (i.e., the blood that was clotted using T-PRF or L-PRF) was processed with a scanning electron microscope (SEM). The other half of each clot was processed for fluorescence microscopy analysis and light microscopy analysis. The T-PRF samples seemed to have a highly organized network with continuous integrity compared to the other L-PRF samples. Histomorphometric analysis showed that T-PRF fibrin network covers larger area than L-PRF fibrin network; also fibrin seemed thicker in the T-PRF samples. This is the first human study to define T-PRF as an autogenous leukocyte- and platelet-rich fibrin product. The platelet activation by titanium seems to offer some high characteristics to T-PRF.