High-Throughput Analysis of Ovarian Granulosa Cell Transcriptome

Table 1

Granulosa cell gene expression profiling in non-human species (↑ and ↓ denote up- and downregulated genes, resp.).

Species	Type of cell	Quantity of material analysed	Kind of assay	Gene profiles/outcome	Reference
Mouse	Preovulatory granulosa cells from wild-type and ER𝛽-null mice	Captured cells from 2-3 animals of the same genotype were pooled, with at least 3 pools collected per cell type	Affymetrix Mouse Genome 430 2.0 GeneChip arrays	Tigd3, Ak7, Arnt2, Nid2, Eya4, Mbl2, Ooep, Ptgis, Oog1, Pou5f1, Trim61, and  Tmem182↑ in PMSG primed ERβ-null mice granulosa cells versus PMSG primed wild-type mice granulosa cells.  Epha5, Ank1, Me2, Reln, Cbs, Cdh2, Ces1d, Mpp7, an  Susd4↓ in PMSG primed ERβ-null mice granulosa cells versus PMSG primed wild-type mice granulosa cells	Binder et al., 2013 [11]
Mouse	CCs from noncompetent antral oocytes	Total 638 COCs	Illlumina Sentrix arrays	Has2, Ptx3,  Tnfaip6, and Ptgs2↓  Amh↑; in noncompetent versus competent oocytes	Vigone et al., 2013 [12]
	CCs from competent antral oocytes	Total 1769 COCs
Rat	Mural granulosa cells from; normal developmental competence oocyte-collected follicles (NDC)	4 samples	Affymetrix GeneChip arrays Rat 230.2.	Lox, Ngfrap1↑ Ggbt2 ↓; in NDC versus PDC	Jiang et al., 2010 [13]
	Mural granulosa cells from; poor developmental competence oocyte-collected follicles (PDC)	4 samples
Pig	Small, medium, and; large antral follicles	Pooled granulosa cells from follicles of three different size classes. Four samples from small follicles, five samples from medium and large follicles	Microarray	Hadhb, Psmc2, Gsta1, Ctsl, Hspa8, Mgst1, Erp29, Gart, Cyb5, and   Cct1↑  in large follicles versus small and medium follicles; Gsto1, Pkm2, Tuba1b, Calu,  Cfl1,  Dag1, Tubb5, Eef1a, and Rps17↑  in small follicles versus medium and large follicles	Bonnet et al., 2008 [10]
Bovine	Cumulus cells from adult and prepubertal; oocytes	Pools of cumulus cells (𝑛=4) collected from adult and prepubertal animals	Bovine cDNA array (Gene Expression Omnibus platform GPL325)	Ctsb, Ctss, and  Ctsz↑; in cumulus cells collected from oocytes with low developmental competence versus those collected from oocytes with high developmental competence	Bettegowda et al., 2008 [14]
Bovine	COCs from 3 to 8 mm follicles	4 samples	Homemade microarray performed using the VersArray ChipWriter Pro	Has2, InhbA, Egfr, Grem1, Btc, CD44, Tnfaip6, and  Ptgs2, markers of oocyte competence, expressed in CCs	Assidi et al., 2008 [15]