Potential Probiotic Escherichia coli 16 Harboring the Vitreoscilla Hemoglobin Gene Improves Gastrointestinal Tract Colonization and Ameliorates Carbon Tetrachloride Induced Hepatotoxicity in Rats

Table 1

Bacterial strains and plasmids.

Plasmids/strains	Relevant characteristics	Reference
Plasmids
pUC-gfp	Derived from the high-copy number vector pUC18 by insertion of a modified gfp gene; Ap^r	[26]
pUC8:16	Derived from the high-copy number vector pUC8 by insertion of a vgb gene; Ap^r	[19]
pUC8:16-gfp	Derived from the high-copy number vector pUC8:16 by insertion of a gfp gene; Ap^r	This study
Bacterial strains
E. coli DH5α	F-endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG Φ80dlacZΔM15Δ(lacZYA-argF)U169, hsdR17 (rK− mK+), λ−	[24]
E. coli BL21	F ′ ompT hsdSB (rB−  mB−)  gal  dcm	[24]
E. coli 16	Wild type	[13]
E. coli 16 (pUC-gfp)	E. coli 16 with pUC-gfp plasmid; Ap^r	This study
E. coli 16 (pUC8:16-gfp)	E. coli 16 with pUC8:16-gfp plasmid; Ap^r	This study

Ap^r represents ampicillin resistance; gfp represents green fluorescent protein; pUC8:16-gfp represents construct containing vgb gene tagged with gfp.