Histone Deacetylase Inhibitor Impairs Plasminogen Activator Inhibitor-1 Expression via Inhibiting TNF-α-Activated MAPK/AP-1 Signaling Cascade

Figure 2

Effect of CBHA on TNF-α-activated signalings in MeT-5A cells. (a) Cells were pretreated with vehicle, PD98059 (PD, 20 μM), LY294002 (LY, 10 μM), Parthenolide (Par, 10 μM), SP600125 (SP, 10 μM), and SB203580 (SB, 20 μM), and then stimulated with TNF-α (10 ng/mL) for 24 h. PAI-1 protein expression was assessed by Western blot. (b) and (c) Cells were treated with TNF-α for the indicated times (upper panel) or pretreated with vehicle or CBHA (0.5–2 μM) for 15 min followed by TNF-α administration (lower panel). Phosphorylation of (b) p38 and (c) JNK MAPKs was analyzed by Western blotting with antibodies specific for either phosphorylated or total proteins.

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