Local Overexpression of V1a-Vasopressin Receptor Enhances Regeneration in Tumor Necrosis Factor-Induced Muscle Atrophy

Figure 1

Expression of the transfected cDNA constructs is increased in electroporated muscles. Real-time PCR analysis of V1aR (a) and TNF (b) was performed on RNA extracts obtained from WT, mock-, V1aR-, TNF-, and V1aR+TNF-transfected TA muscles to verify the transfection efficiency after electroporation. The expression of V1aR and TNF is not significantly different when comparing V1aR versus V1aR+TNF and TNF versus V1aR+TNF samples, respectively (Figures 1(a) and 1(b)). Values represent the average three independent experiments. ^∗𝑃<0.05; ^∗∗𝑃<0.01; ^∗∗∗𝑃<0.001 by Student’s 𝑡-test.

(a)

(b)