Phenotypic and Genotypic Characterization of Enterotoxigenic Escherichia coli Clinical Isolates from Northern Colombia, South America

Table 5

Characterization of the 6 ETEC clonal groups based on MLST, serotype, classical and nonclassical virulence factors, and antibiotic pattern.

Isolate	Classical virulence factors^a			Non-classical virulence factors	Clonal group^e	SeqT group^f	Serotype	Antibiotic resistance^h
	Enterotoxins		CS^c
	ST^b	LT
COCt122	+	−	CFA/I, CS21	eatA, irp2, fyuA, etpA, etpB	1	2332	O128:H45	AM, STX
COCt129	+	−	CFA/I, CS21	eatA, irp2, fyuA, etpA, etpB	1	2332	O128:H45	AM, STX
COCt142	+	−	CFA/I, CS21	eatA, irp2, fyuA, etpA, etpB	1	2332	O128:H45	AM, STX
COCt249	+	−	CFA/I, CS21	eatA, irp2, fyuA, etpA, etpB	1	2332	O128:H45	AM, STX
COCt253	+	−	CFA/I, CS21	eatA, irp2, fyuA, etpA, etpB	1	2332	O128:H45	AM, STX
COCt310	+	−	CFA/I, CS21	eatA, irp2, fyuA, etpA, etpB	1	2332	O128:H45	AM, STX, CZ
COCt140cc	+	−	CFA/I, CS21	eatA, irp2, fyuA, etpA, etpB	1	2332	O128:H45	AM, STX, AMC
COCt124	+	+	CS5, CS6	eatA, tia	2	443^g	O167:H5	ND
COCt201	+	+	CS5, CS6	eatA, tia	2	443^g	O167:H5	AM, STX
COCt235	+	+	CS5, CS6	eatA, tia, irp2, fyuA	2	443^g	O167:H5	AM, STX
1COSc36	+	+	CS5, CS6	eatA, tia	2	443^g	O167:H5	AM, STX
COCt 161	+	−	ND^d	irp2, fyuA	3	38	O153:H18	AM, STX, CZ
COCt 285	+	−	CFA/I, CS21	eatA, irp2, fyuA, etpA, etpB	3	38	O153:H18	AM, STX
1COCt40	−	+	CS6, CS21	eatA, irp2, fyuA	4	1312	O25:H16	AM, STX, CZ
1COSc61	−	+	CS6, CS21	eatA, irp2, fyuA	4	1312	O25:H16	AM, STX, CZ
COCt200	+	−	CS21, CFA/I	eatA, irp2, fyuA, etpA, etpBirp2, fyuA	5	88	O128:H45	AM, STX
1COCt43	−	+	CS18		5	88	O8:H9	AM, STX, CZ
COCt131	−	+	CS7, CS21	eatA, tibA, irp2, fyuA, etpA, etpB	6	137	O78:H10	AM, STX
COCt337	−	+	CS7	eatA, tibA, irp2, fyuA, etpA, etpB	6	137	O(−):H(−)	AM, STX, CZ

aST, heat-stable toxin; heat-labile toxin.
^bAll ETEC TS positives were positive for the STh variant.
^cCS refers to colonization surface antigens.
^dND: not detected.
^eBased on phylogenetics (see Section 2).
^fAs determined by EcMLST (http://mlst.ucc.ie/mlst/dbs/Ecoli).
^gETEC strains with SeqT 443 that contain a single icd locus variant. The icd gene has 517/518 matches (mutation in T-229).
^hResistance to AM, ampicillin; STX: sulfamethoxazole; CZ: cefazolin; AMC: amoxicillin /clavulanic acid.