CpG Island Methylator Phenotype and Prognosis of Colorectal Cancer in Northeast China

Table 1

HRM primers and amplicon information.

Gene name	Primer sequences 5′-3′	GenBank	Number of CpG-sites/length
		Accession number	of amplified fragment
MGMT	Fw GCGTTTCGGATATGTTGGGATAGT; Rv AACGACCCAAACACTCACCAAA	X61657	15/110
MLH1	Fw TTTTTTTAGGAGTGAAGGAGG; Rv AACRCCACTACRAAACTAAA	AY217549	13/123
APC	Fw AAGTAGTTGTGTAATTCGTTGGAT; Rv CACCTCCATTCTATCTCCAATA	NT_034772	11/149
p16	Fw GGAGTTTTCGGTTGATTGGTTGGTT; Rv AACAACGCCCGCACCTCCTCTA	AF527803	5/69
MINT1	Fw GGGGTTGAGGTTTTTTGTTAG; Rv AATCCCTCTCCCCTCTAAACTT	AF135501	7/137
MINT31	Fw GGGTGATGGTTTTAGTAAAGTGAG; Rv AAAAACACTTCCCCAACATCTAC	AF135531	10/164
RUNX3	Fw TTTTTAGAGAATGAGGGATTTTTGT; Rv CCCTAATCCCTTAAATCTAATACCC	NC_000001	7/115