MicroRNA Dysregulation in Liver and Pancreas of CMP-Neu5Ac Hydroxylase Null Mice Disrupts Insulin/PI3K-AKT Signaling
Figure 3
Expression changes in the insulin/PI3K-AKT signaling pathway. (a) Expression levels of IRS1, IRS2, AKT1, and FOXO1 mRNAs in livers (left) and pancreas (right) of control and Cmah-null mice were determined using real-time reverse transcription PCR (RT-qPCR) (; ; ). (b) Western blot analysis of proteins involved in the target pathway in liver (left) and pancreas (right) of control and Cmah-null mice. Actin was used as a loading control. Band intensities were quantified by image processing and analysis was made using ImageJ v1.32.
