Extracts from Glioma Tissues following Cryoablation Have Proapoptosis, Antiproliferation, and Anti-Invasion Effects on Glioma Cells
Figure 1
Apoptosis analysis in glioma tissues following cryoablation. (a) Apoptosis in glioma tissues at 8 h after cryoablation for three cycles of rapid freezing/thawing detected via the TUNEL assay. Scale bar, 50 m. (b) Apoptosis in glioma tissues at 24 h after cryoablation for one cycle of rapid freezing/thawing detected via the TUNEL assay. Scale bar, 100 m. (c) The comparison of the levels of the rapid-onset apoptosis and the delayed apoptosis in glioma tissues at different time points after cryoablation for one cycle of rapid freezing/thawing. (d) The levels of PARP, procaspase-8, procaspase-9, and their cleaved forms in the four sections of tumor tissues at 24 h after cryoablation for one cycle of rapid freezing/thawing were analyzed via Western blot. S1–S4, Sections 1–4, in a tumor with the treatment of cryoablation: S1, the center of frozen tissues, S2, peripheral region of frozen tissues, S3, areas outside the frozen tissues, and S4, normal tissues of mice surrounding the tumor.
