Adhesion of Pancreatic Cancer Cells in a Liver-Microvasculature Mimicking Coculture Correlates with Their Propensity to Form Liver-Specific Metastasis In Vivo

Figure 8

Adhesion of BxPC-3 (a) and MIA-PaCa-2 (b) in the TMNK-1 only and TMNK-1 + 10T1/2 in the collagen gel cultures upon blocking of VAP-1 ((a1) and (b1)) and ICAM-1 ((a2) and (b2)). In the VAP-1 blocking experiment, two peptides were used: GGGGGGGGK (P1, as control) and GGGGKGGGG (P2, effective peptide), which bound poorly and efficiently with VAP-1, respectively [3]. ICAM-1 was blocked with anti-ICAM-1 antibody, while control was an isotype IgG antibody. Columns and error bars represent mean − SD of two to three independent experiments. Each independent experiment had four to six wells for each culture condition. Statistical significance is shown using symbols ^∗(𝑃<0.05), ^∗∗(𝑃<0.01), and ^∗∗∗(𝑃<0.001).