Endothelium-Independent Vasorelaxant Effects of Hydroalcoholic Extract from Nigella sativa Seed in Rat Aorta: The Roles of Ca²⁺ and K⁺ Channels

Figure 5

Effect of cumulative concentrations of Nigella sativa seed extract (2, 4, 6, 8, 10, and 14 mg/mL) on rat intact aortic rings contracted with PE (10⁻⁶ M) (PE + E), after pretreatment with glibenclamide (10⁻⁵ M) (PE + Gly) or tetraethylammonium chloride (5 mM) (PE + TEA). Data are expressed as mean ± S.E.M. (𝑛=8). ^∗𝑃<0.05, ^∗∗𝑃<0.01, and ^∗∗∗𝑃<0.001 compared to PE + E; ⁺𝑃<0.05, ⁺⁺𝑃<0.01, and ⁺⁺⁺𝑃<0.001 compared to PE + TEA.