Pu-Erh Tea Extract Induces the Degradation of FET Family Proteins Involved in the Pathogenesis of Amyotrophic Lateral Sclerosis
Figure 5
Lysosome-dependent degradation of FUS/TLS by PTE. SK-N-SH cells were incubated with or without inhibitor for 1 h, (a) ammonium chloride (NH4Cl), a lysosome inhibitor, or (b) lactacystin, a proteasome inhibitor, followed by treatment with PTE (200 μg/mL) for 24 h. Cell lysates were detected by Western blot with anti-FUS/TLS. β-actin was used as the loading control. (c) (d) The Western blot results were quantified and statistical analysis was performed. Values are mean ± SEM of three independent experiments. *, **, and *** compared with treated with PTE only.