Research Article

Epinephrine Enhances the Response of Macrophages under LPS Stimulation

Table 1

RT-PCR primers.

PrimerTypeSequenceProduct size (bp)

GAPDHSense5′-GCAAGTTCAATGGCACAGTCAAGG-3′471
Antisense5′-TGGATGCAGGGATGATGTTCTGG-3′

CD14Sense 5′-CACAAATTCCCGACCCTCCAAGT-3′425
Antisense5′-AAAGTTCCTGACAAGCCCGCTGA-3′

All primers were synthesized by Sangon (Shanghai, China) with the catalog numbers NM017008 and NM021744, respectively. PCR parameters for rat GAPDH and CD14 proceeded as follows: 94°C for the initial 3 min; 27 cycles of 94°C for 30 sec, 60°C for 30 sec, and 72°C for 40 sec; and 72°C for the final 2 min.