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BioMed Research International
Volume 2014 (2014), Article ID 254863, 10 pages
http://dx.doi.org/10.1155/2014/254863
Research Article

Morphogenesis and Production of Enzymes by Penicillium echinulatum in Response to Different Carbon Sources

Enzyme and Biomass Laboratory, Institute of Biotechnology, University of Caxias do Sul, 1130, Francisco Getúlio Vargas Street, 95070-560 Caxias do Sul, RS, Brazil

Received 15 February 2014; Accepted 4 April 2014; Published 30 April 2014

Academic Editor: Brad Upham

Copyright © 2014 Willian Daniel Hahn Schneider et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The effect of different carbon sources on morphology and cellulase and xylanase production of Penicillium echinulatum was evaluated in this work. Among the six carbon sources studied, cellulose and sugar cane bagasse were the most suitable for the production of filter paper activity, endoglucanases, xylanases, and β-glucosidases. However, sucrose and glucose showed β-glucosidase activities similar to those obtained with the insoluble sources. The polyacrylamide gels proved the enzymatic activity, since different standards bands were detected in the media mentioned above. Regarding morphology, it was observed that the mycelium in a dispersed form provided the greatest enzymatic activity, possibly due to greater interaction between the substrate and hyphae. These data are important in understanding the physiology of fungi and could contribute to obtaining enzyme with potential application in the technology of second generation ethanol.