Research Article
A Founder Large Deletion Mutation in Xeroderma Pigmentosum-Variant Form in Tunisia: Implication for Molecular Diagnosis and Therapy
Table 3
Complete list of primers used to gDNA amplification of exon 10 and its intronic boundaries.
| | Name | Sequence 5′→3′ | Annealing Temperature (°C) | Suspected PCR products size (bp) | PCR Product size for XP-V patients (bp) |
| | XPV10F | CCTGGTTCTTTTAATTTCCTCTCCTG |
55 |
459 |
— | | XPV10R | CATTTACCCTTTACCTCATTGAAGGAC |
| | XPV del 10 F | TCATTTGTGCTGTCCTGTTC |
60 |
3012 |
— | | XPV del 10 R | GGTTGCAGTGAGCGGAGATT |
| | Del ex10 LR-F | AGGTCCTCCCTAGTTACCCTATCACAGCAG |
60 |
4105 |
— | | Del ex10 LR-R | ACTACCTAACCCTGACTGACTTACCACTCTGG |
| | POLH10ΔF | AGTGGGTAGGTTTTGGTAGCTGTGGAAG |
60 |
9358 | ≈6000 bp | | POLH10ΔR | GGACACACCCTGGATACTCTGTTGGTAA |
| | POLHFΔ | ACCTTGGAGTATAATTTCTGGGTCA |
59 |
5212 | ≈1000 bp | | POLHRΔ | GTCATAAAGTTCCTCATTGTGTCTAA |
| | POLHdelF | CATGTGCTTGTTGGACATTTG |
60 |
4526 | ≈500 bp | | POLHdelR | GGTTTCATGCTTTGGGACAG | |
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