Research Article

Purification and Characterization of Alkaline-Thermostable Protease Enzyme from Pitaya (Hylocereus polyrhizus) Waste: A Potential Low Cost of the Enzyme

Table 1

Purification step of the thermoalkaline protease from Hylocereus polyrhizus peel.

Purification stepsTotal protein (mg)Total activity (U)Specific activity (U/mg)Purification foldYield (%)

Crude extract44.2557.212.61100
Ammonium sulphate precipitation 3.9462.4118.49.483.2
Cation exchange chromatography0.3412.81312.9104.274.1
Gel filtration chromatography0.1397.22787.1221.271.3

Fold purification calculated with respect to the specific activity of the crude extract.