Effects of Crude Extracts from Medicinal Herbs Rhazya stricta and Zingiber officinale on Growth and Proliferation of Human Brain Cancer Cell Line In Vitro
CAERS and CFEZO treatments trigger mitochondrial-dependent apoptotic pathway. U251 cells were treated with indicated concentrations of CAERS, CFEZO, and combination of CAERS CFEZO for 24 h and assayed as detailed in Section 2. (a) Immunoblots showing CAERS and/or CFEZO treatments mediated mitochondrial cyto c release, activation of caspases 9 and 3, and PARP-1 cleavage. The mitochondrial marker, anticytochrome oxidase IV (COXIV), shows the purity of the cytoplasmic fraction and equal loading of the mitochondrial fraction. (b) CAERS and/or CFEZO treatment(s) altered expression ratio of Bax/Bcl-2 at protein and mRNA levels, in favor of apoptosis. The histogram depicts the Bcl-2 (dark bars) and Bax (light bars) mRNA ratio measured by using densitometric analysis. In all western blot analyses, the membranes were stripped and reprobed with antiactin antibody as a loading control.