Protective Role of 5-Lipoxigenase during Leishmania infantum Infection Is Associated with Th17 Subset
Figure 4
The absence of 5-LO affected the cytokine release related to Th17 pattern. The spleen cells from the WT and mice at the 6th week pi or uninfected were in vitro stimulated with the L. infantum antigen (50 μg/mL) or medium for 72 hours, and the levels of IFN- (a), IL-17 (b), TNF (c), IL-23 (d), IL-6 (e), IL-1β (f), and TGF-β (g) were measured in the culture supernatants by ELISA assay. The data are expressed as the mean ± SEM and one representative of two independent experiments. compared to the medium; compared with the WT stimulation. The liver fragments from the WT (white bar) or (black bar) at the 6th wpi with the L. infantum promastigote forms were collected and weighed for the determination of IFN- (h), IL-17 (i), TNF (j), and IL-12p40 (k) by ELISA in the homogenate supernatants. The data are expressed as the mean ± SEM, N = 5-6. compared with the WT group.
