Table of Contents Author Guidelines Submit a Manuscript
BioMed Research International
Volume 2014, Article ID 269253, 9 pages
http://dx.doi.org/10.1155/2014/269253
Research Article

Effect of Equal Daily Doses Achieved by Different Power Densities of Low-Level Laser Therapy at 635 nm on Open Skin Wound Healing in Normal and Diabetic Rats

11st Department of Surgery, Pavol Jozef Šafárik University, Medical Faculty and Louis Pasteur University Hospital, Trieda SNP1, 040 11 Košice, Slovakia
2Department of Medical and Clinical Biophysics, Pavol Jozef Šafárik University, Medical Faculty, Košice, Slovakia
3Department of Cardiovascular Diseases, International Clinical Research Center, St. Anne’s University Hospital and Masaryk University, Pekarska 53, 656 91 Brno, Czech Republic
41st Department of Internal Medicine, Pavol Jozef Šafárik University, Medical Faculty and Louis Pasteur University Hospital, Košice, Slovakia
5Department of Surgery, Pavol Jozef Šafárik University, Medical Faculty and 1st Private Hospital, Košice-Šaca a.s., Košice, Slovakia
6Department of Forensic Medicine, Pavol Jozef Šafárik University, Medical Faculty, Košice, Slovakia

Received 11 October 2013; Revised 14 December 2013; Accepted 14 December 2013; Published 16 January 2014

Academic Editor: Marília Gerhardt De Oliveira

Copyright © 2014 Róbert Kilík et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background and Objective. Despite the fact that the molecular mechanism of low-level laser therapy (LLLT) is not yet known, the exploitation of phototherapy in clinical medicine and surgery is of great interest. The present study investigates the effects of LLLT on open skin wound healing in normal and diabetic rats. Materials and Methods. Four round full-thickness skin wounds on dorsum were performed in male adult nondiabetic () and diabetic () Sprague–Dawley rats. AlGaInP (635 nm, wavelength; 5 J/cm2, daily dose) was used to deliver power densities of 1, 5, and 15 mW/cm2 three times daily until euthanasia. Results. PMNL infiltration was lower in the irradiated groups (15 mW/cm2). The synthesis and organisation of collagen fibres were consecutively enhanced in the 5 mW/cm2 and 15 mW/cm2 groups compared to the others in nondiabetic rats. In the diabetic group the only significant difference was recorded in the ratio PMNL/Ma at 15 mW/cm2. A significant difference in the number of newly formed capillaries in the irradiated group (5, 15 mW/cm2) was recorded on day six after injury compared to the control group. Conclusion. LLLT confers a protective effect against excessive inflammatory tissue response; it stimulates neovascularization and the early formation of collagen fibres.